Retention of dRBCs by the isolated-perfused human spleen and the microsphiltration system. (A) Kinetics of dRBC clearance in the perfused spleen, monitored by measuring PKH-labeled counts in the perfusate (a representative experiment, n = 8). (B) Linear regression fit of the correlation between levels of dRBC retention within the spleen and the relative rigidity. (C) Retention rates of dRBCs in the isolated-perfused human spleen 2 hours after the onset of perfusion or in the microspheres filter. (D) Linear regression fit of the correlation between levels of dRBC retention within the microsphilters and the relative rigidity.