Dimensional and morphological characteristics of dRBCs. Projected surface area (A), diameter (B), circularity (C), aspect ratio (D), and shape ratio (E) (sphericity) of untreated RBCs and RBCs treated with increasing concentrations of diamide (10-500 µM) and analyzed with an ImageStream imaging cytometer. RBCs treated with LPC to induce a control loss of cell surface area⁵  were used as a positive control for imaging. Unlike LPC treatment, exposure to diamide had no effect on RBC dimensions (surface area and diameter [A-B]) and only a slight effect on cell morphology (circularity [C] and sphericity [D-E]).