NLRP3 and ASC colocalize in platelets derived from SCD patients. (A) In platelets derived from SCD patients in steady state, intraplatelet ASC (red) colocalizes with NLRP3 (green), as demonstrated with immunofluorescence staining and confocal laser scanning microscopy. Images show maximum intensity projection of confocal images Z-stacks and are representative of 3 independent preparations per group. Scale bar, 2 μm. (B) Quantification of NLRP3/ASC colocalization using the Pearson correlation coefficient. Data are shown as median with IQR and minimum and maximum values from n = 3 subjects per group. The P value was calculated using the Mann-Whitney U test.