Overexpression of KDM6B in BM cells led to epigenetic activation of innate immune genes. (A) Quantitative reverse transcription reverse transcription-PCR results showing mRNA expression of the S100a9 gene in mouse BM cells (N = 10 Vav-KDM6B, N = 13 WT) (B) Chromatin immunoprecipitation-PCR results showing H3K4me3 at the regulatory region of the S100a9 gene in mouse BM cells (N = 5 in each group). (C-D) Quantitative reverse transcription-PCR results showing mRNA expression of innate immune genes TLR1 and C3 in mouse BM cells (treatment with LPS: N = 10 Vav-KDM6B, N = 8 WT: treatment with PBS: N = 12 Vav-KDM6B, N = 13 WT). (E-F) Chromatin immunoprecipitation-PCR results showing promoter H3K4me3 at the regulatory regions of the TLR1 and C3 genes (N = 5 in each group). All error bars are based on ± SEM.