Overexpression of KDM6B in Vav-KDM6B mice led to moderately impaired hematopoiesis and bone marrow dysplasia. (A) Relative mRNA expression levels of human KDM6B in bone marrow (BM) cells of adult Vav-KDM6B (N = 16) and WT (N = 12) mice. (B) Complete blood count results for WBCs and neutrophils of adult Vav-KDM6B mice (N = 46) compared with littermate WT controls (N = 27). (C) Kaplan-Meier survival curves of Vav-KDM6B (N = 24) and WT (N = 18) mice. (D) WBC counts in old (age > 70 weeks) Vav-KDM6B mice (N = 19) and age-matched WT controls (N = 15) compared with young (age 20-30 weeks) mice. (E) Example images of BM cytospin showing megakaryocytic dysplasia and in old Vav-KDM6B mice (N = 6) and age-matched WT controls (N = 4). Scale bars represent 15 μm. (F) Primary (G1) and secondary (G2) plating colony-forming units in serial methylcellulose colony formation assays of lineage-negative, cKit-positive, Sca1-positive (LSK) BM cells isolated from old, age-matched WT (N = 9) and Vav-KDM6B (N = 13) mice. (G) Kinetics of peripheral blood WBC counts in recipient mice transplanted with LSK BM cells of old, age-matched WT (N = 4) and Vav-KDM6B (N = 7) mice. (H-I) Percentages of LSK BM cells and Gr1⁺ cells in recipient mice transplanted with LSK BM cells of old Vav-KDM6B (N = 7) and age-matched WT (N = 4) mice. All error bars are based on ± SEM.