Histopathologic infiltration of CD8⁺T cells and production of proinflammatory cytokines and chemokines in A20 tumors are markedly increased after accelerated LTI. (A) Immunohistochemistry of A20 tumors that were untreated or treated with conventional or accelerated LTI starting on day 21. Tissue sections obtained from day-27 tumors were stained with anti-CD3, anti-CD4, and anti-CD8 mAbs conjugated with fluorochromes using a 2-stage procedure. CD8⁺ is red, CD4⁺ is blue, and CD3⁺ is green. (B) Lysates from tumor tissues were analyzed for expression of various cytokines and chemokines by Luminex-based assay with or without LTI treatment. Concentrations of cytokines and chemokines per 100 μg of total protein. Data are mean ± SEM (n = 5 mice per group). Only significant P values are shown. *P < .05, **P < .01, Mann-Whitney U test.