Analysis of platelet adhesion and secretion, as well as hemostasis, in Tln1^R35E/R35Emice. (A) P-selectin (CD62P) surface expression onto platelets in whole blood. Bar graph represents ΔMFI ± SEM (n = 4 mice, representative of 2 independent experiments). (B-C) Adhesion of Tln1^R35E/R35E platelets onto fibrinogen-coated surfaces. Washed platelets were stimulated with thrombin (0.01 U/mL). Platelets were pooled from 3 mice, 2 independent experiments. (B) Bar graphs are mean ± SEM of adhered platelet counts at the indicated time points after stimulation. (C) Quantification of platelet area at 45 minutes. Data are mean ± SEM. (D) Tail bleeding analysis. The experiment was terminated at the end of 10 minutes to avoid excessive loss of blood. No significant differences were observed between Tln1^wt/wt mice (n = 15) and Tln1^R35E/R35E mice (n = 14).