Unstable aggregation of Tln1^R35E/R35Eplatelets in response to stimulation with a low dose of agonists. Aggregation of Tln1^wt/wt and Tln1^R35E/R35E platelets stimulated with various doses of PAR4-AP (A), fibrillar collagen type I (B), and the thromboxane A2 analog U46619 (C). Arrows indicate addition of the agonist. Results are representative of 3 independent experiments, n = 3 mice each time.