Interaction between endothelial SDC-1 and VWF fibers. Blood vessel of tumor tissue generated by control melanoma cells (shCTL) (A) or melanoma cells (B) with silenced HPSE (shHPSE). (A-B) White boxes mark the area of the depicted magnifications shown below. Scale bars, 20 µm. SDC-1 is shown in red, VWF in green, and nuclei in blue. (C) Quantifications of the endothelial SDC-1 (eSDC-1) and the total VWF content in shCTL and shHPSE tumors (n = 4). Signal levels were expressed as mean fluorescence intensity (MFI). (D) Change of VWF fiber amount retained at the blood vessel wall in shCTL and shHPSE tumors (n = 4). Differences in signal levels were expressed as change in MFI (ΔMFI). *P ≤ .05, **P ≤ .01 (Student t test).