PEG-RBCs fail to bind to transiently transfected CHO cells.

Control and PEG-modified RBCs were assessed for binding to CHO cells transiently expressing the glycophorin A binding domain of the P falciparum protein ligand, EBA-175. There is a dose-dependent inhibition of rosetting using RBCs treated with varying concentrations of both the 3.35 kd PEG derivative (■) and the 18.5 kd PEG derivative (▪). Error bars indicate the standard deviation of 3 determinations. Percentage inhibition of rosetting was determined by comparing PEG-modified RBC rosettes to control RBC rosettes as follows: Control RBC rosettes − PEG RBC rosettes/Control RBC rosettes × 100.