sph^Dem/sph^Demmice have severe HE.

(A) Wright-stained peripheral blood smears from +/+ (left panel) andsph^Dem/sph^Dem (right panel) mice. Note the presence of spherocytic, elliptocytic, poikilocytic, and fragmented RBCs in the mutant mice. Bar, 5 μm. (B) Scanning electron microscopy of RBCs from +/+ (left panel) andsph^Dem/sph^Dem (right panel). Note the abnormally shaped RBCs in the mutant. Bar, 1 μm. (C) Osmotic deformability profiles of RBCs from wild type (+/+), heterozygous (sph^Dem/+), and homozygous (sph^Dem/sph^Dem) mice.