Characterization of protein A-purified IgG from pooled plasma of untreated and vitamin E-treated apoE^−/− mice.

The 2 principal IgG preparations contained no β₂GP1. (A) Increasing concentrations of IgG (from vitamin E-treated plasma), which contained no β₂GP1, were added to microtiter wells containing a fixed amount of OxCL and the amount of IgG bound measured as described in “Materials and methods.” (B) A fixed content of purified IgG (from untreated plasma) was added to microtiter wells containing an increasing concentration of either OxCL or a “reduced” CL analog unable to undergo oxidation. Each point is the mean of triplicate determinations.