Induction of apoptosis in KG1 cells overexpressing thehiwi gene.

KG1 cells were transfected under mock, pCIneo empty vector, or pCIneo-hiwi conditions. Cells were harvested at 2, 8, 12, and 32 hours, washed with PBS, and resuspended in binding buffer containing Annexin V–FITC and PI. The X-axis represents log fluorescence intensity for Annexin V staining; the Y-axis represents log fluorescence intensity for PI staining. Panels A-C show flow cytometric analysis at a 2-hour time point that consists of a mock, a pCIneo empty vector, and the pCIneo-hiwi transfected KG1 cell populations. Panels D-F, G-I, and J-L show the same analysis but at 8,12, and 32 hours (respectively) after transfection.