PCR detection of hiwi DNA in transfected KG1 cells.

(A) PCR amplification of hiwi on mock transfected KG1 cells (d1-6, d9; lanes 2-8), empty vector transfected KG1 cells(d1-6, d9; lanes 9-15), and hiwi-containing vector-transfected KG1 cells (d1-6, d9; lanes 16-22). Lane 1 is a 1-kb DNA ladder. (B) Samples without reverse transcriptase; same setup as panel A. (C) GAPD internal control (142 bp), same setup as panel A.