Loss of

hiwi expression during differentiation of CD34⁺ marrow cells. CD34⁺ cells were placed in suspension culture with G-CSF, IL-3, and SCF at 100 ng/mL added every 3 to 4 days. (A) Expression of hiwi as detected by semiquantitative RT-PCR. Lane 1, 123-bp DNA ladder; lane 2, day 0 CD34⁺ cells; lane 3, day 0 CD34⁻cells; lane 4, day 1 culture sample; lane 5, day 3 culture sample; lane 6, day 5 culture sample; lane 7, day 7 culture sample; lane 8, day 10 culture sample; lane 9, day 14 culture sample; lane 10, negative control (water); lane 11, positive control (human testis, 269 bp). (B) Internal control of β₂-microglobulin gene expression throughout 14 days of culture (330 bp). Lane numberings correspond to the same samples as in panel A.