Fig. 2.
![Fig. 2. PKC assay. / PKC activity was assayed by quantitation of 32P incorporation from [γ32P]-ATP into the substrate, MARCKSPSD peptide, by using lysates from HMVEC-Ls treated with Tat for the indicated times. The 32P-incorporated substrates were precipitated with TCA plus BSA, and the amount of radioactivity in the precipitates was measured by Cherenkov counting.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/2/10.1182_blood.v97.2.352/5/h80210602002.jpeg?Expires=1722387555&Signature=T50ukXFhAApOrFWoZmZC7GIz3wKZ~MQTse337zH8fY~QVr7woX33UfBEgb~9z~0HuLca~co0pJQvHTHLNYvtb0OQjMFpUmTCWGb2pxYYUKPUcBm~jXuyefRvHbdkM-C~iCRup6lFWbnyD1fALTBVRfAwfW4UG7oxMDjkhz99hQsiHcRSzUj6nKyD1sA5ERTnc6mgO3TcEbiDqm2sZW7tuWvQvkChWxOH~ERV106RiDWbNVL5Mjqx05myunmM7dBAwF1x0126KKbDIafxiNxSxgsMTL0dD-KrY9NIXEz9ySmbFPaF~KaAn5wrc16FfY4s~YZ1RjJkkQlzPzdlc3WIkQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PKC assay.
PKC activity was assayed by quantitation of 32P incorporation from [γ32P]-ATP into the substrate, MARCKSPSD peptide, by using lysates from HMVEC-Ls treated with Tat for the indicated times. The 32P-incorporated substrates were precipitated with TCA plus BSA, and the amount of radioactivity in the precipitates was measured by Cherenkov counting.
