BMP-4 induces cell cycle arrest in OH-2 and ANBL-6 cells.

OH-2 cells were incubated with 0.1 ng/mL IL-6, with or without 20 ng/mL BMP-4 (upper histograms). ANBL-6 cells were incubated with 1 ng/mL IL-6, with or without 100 ng/mL of BMP-4 (lower histograms). After cytokine stimulation for 18 hours, cells were washed, permeabilized, and stained with PI. Cellular DNA content was measured by flow cytometry. The percentage of cells in the G₀/G₁phase of the cell cycle is indicated in each histogram.