BMP-4 reduces viability of OH-2 and IH-1 cells but not in ANBL-6 cells.

Annexin V–FITC and PI binding were measured by flow cytometry in myeloma cell lines incubated for 72 hours with BMP-4 in concentrations as indicated and 1 ng/mL IL-6 (ANBL-6) or 0.1 ng/mL IL-6 (IH-1 and OH-2). Percentage of viable cells, located in the lower left quadrant of dot plots, as exemplified in Figure 7, was recorded.