American Society of Hematology

Fig. 2.

$Fig. 2. Characterization of HUVEC-secreted and HUVEC-associated proteoglycans and their chondroitinase and heparitinase digestion profiles. / Proteoglycans were purified as described in the text. Intact proteoglycans and the chondroitinase and heparitinase digests from cells and culture medium were chromatographed on the Sepharose CL-6B column. Vo represents the void volume; Vt is the total volume of the column; and the Kav is the fractional distance of migration of the peak between the Voand Vt. The Vo and Vt were marked by blue dextran and phenol red, respectively. Approximately 50 fractions were collected between the Vo and Vt. CONTROL indicates undigested proteoglycans. Within each panel, the same amount of radioactivity was applied to the column for control and digested samples.$

Characterization of HUVEC-secreted and HUVEC-associated proteoglycans and their chondroitinase and heparitinase digestion profiles.

Proteoglycans were purified as described in the text. Intact proteoglycans and the chondroitinase and heparitinase digests from cells and culture medium were chromatographed on the Sepharose CL-6B column. V_o represents the void volume; V_t is the total volume of the column; and the K_av is the fractional distance of migration of the peak between the V_oand V_t. The V_o and V_t were marked by blue dextran and phenol red, respectively. Approximately 50 fractions were collected between the V_o and V_t. CONTROL indicates undigested proteoglycans. Within each panel, the same amount of radioactivity was applied to the column for control and digested samples.

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