IL-10 + TGF-β–tolerant cells synthesize cyclin D2 but not cyclin D3 or cyclin A.

Lysates from unstimulated, tolerant, and primed T cells were analyzed by SDS-PAGE and immunoblotted with an mAb specific for cyclin D2. Blots were stripped and reprobed with an mAb specific for cyclin D3 or a rabbit polyclonal antibody specific for cyclin A.