CD34 and c-Kit expression in BM of mice at various ages.

Expression of c-Kit and CD34 on Lin⁻ BM cells of 1-, 4-, 8-, and 16-week-old mice (Ly-5.2), and the percentages of Ly-5.2⁺ cells in B220⁺ cells (B), Thy-1⁺ cells (T)- and Gr-1/Mac-1⁺ cells (M) in Ly-5.1 recipient PB 8 to 10 weeks after transplantation. The recipients were transplanted with CD34⁻ (R1), CD34^low(R2), and CD34^high (R3) cells (1-5 × 10²[●], 1-5 × 10³ [○], 1-2 × 10⁴[■] and 1 × 10⁵ cells [▵]) sorted from the BM of 1-, 4-, 8-, and 16-week-old mice (Ly-5.2). The averages of the proportions of the 3 fractions in Lin⁻c-Kit⁺cells of 1-, 4-, 8-, and 16-week-old mice (n = 5, 3, 7, and 10) are shown in each window in the flow cytometry graphs.