CD34 expression on HSC in fetal and neonatal liver and spleen.

Expression of c-Kit and CD34 on Lin⁻ BM cells of neonatal liver, neonatal spleen, and fetal liver cells (16 dpc) of Ly-5.2 mice, and the percentages of Ly-5.2⁺ cells in B220⁺cells (B), Thy-1⁺ cells (T), and Gr-1/Mac-1⁺cells (M) in Ly-5.1 recipient PB 8 to 10 weeks after transplantation. The recipients were transplanted with CD34⁻ (R1), CD34^low (R2), and CD34^high (R3) cells (1 × 10² [●], 1 × 10³ [○], 2 × 10³ [▪], 1 × 10⁴ [■] and 2 × 10⁴ cells [⧫]) in Ly-5.2 neonatal liver, neonatal spleen, and fetal liver cells (14-18 dpc). The averages of the proportions of the 3 fractions in Lin⁻c-Kit⁺cells of neonatal spleen, neonatal liver, and fetal liver cells (n = 2, 2, and 4) are shown in each window in the flow cytometry graphs.