Quantitative analysis at different stages of development.

(A) Northern blot analysis of poly(A)⁺ mRNA from whole mouse embryos and embryonic organs at different stages of development and normal adult mouse tissues showing the distribution ofmuIfnar-2 mRNA isoforms. The blots were sequentially hybridized with a full-length muIfnar-2c cDNA probe and aGAPDH probe. (B) Quantitation of muIfnar-2 mRNA expression during murine development. Quantitative analysis of Northern blot expression of muIfnar-2a relative tomuIfnar-2c in whole mouse embryos and embryonic organs at different stages of development, and normal adult mouse tissues. Data are representative of replicate experiments.