Quantitiation of

muIfnar-2 mRNA expression in normal adult mouse tissues, primary cells and cell lines. (A) Quantitative analysis of Northern blot expression of muIfnar-2a relative toGAPDH. Northern blots of poly(A)⁺ mRNA (3 μg) from a representative range of mouse tissues, primary cells, and cell lines were simultaneously electrophoresed then transferred to membranes. The blots were sequentially hybridized with a full-lengthmuIfnar-2c cDNA probe and a GAPDH probe and then exposed to a phosphor screen. The radioactivity in each band hybridizing to the muIfnar-2c and the GAPDHprobes were quantified using a Phosphorimage analyzer with MacBAS v2.5 software. (B) Quantitative analysis of Northern blot expression ofmuIfnar-2c relative to GAPDH. Northern blots of poly(A)⁺ mRNA (3 μg) from a representative range of mouse tissues, primary cells, and cell lines were simultaneously electrophoresed then transferred to membranes and analyzed as described previously. (C) Quantitative analysis of Northern blot expression ofmuIfnar-2a relative to muIfnar-2c. Northern blots of poly(A)⁺ mRNA (3 μg) from mouse tissues, primary cells, and cell lines were hybridized with full-lengthmuIfnar-2c cDNA probe and then exposed to a phosphor screen and quantitated as described previously. Where indicated by error bars, data are the mean ± SEM of at least 3 replicate experiments.