NAC improves survival following induction of acute thrombosis in mice transplanted with JAK2^V617Fcells. Control (WT:WT) and JAK2^V617F engrafted (VF:WT) mice were pretreated with aspirin (50 mg/kg) or NAC (400 mg/kg) for 1 hour, and then treated with decreasing concentrations of a mixture of collagen and epinephrine via retro-orbital injection. (A-C) Kaplan-Meyer curve representing survival of mice injected with collagen and epinephrine concentrations of 0.8 mg/kg and 6 mg/kg (A), 0.4 mg/kg and 3 mg/kg (B), and 0.2 mg/kg and 1.5 mg/kg (C), respectively. (D-G) Representative hematoxylin and eosin–stained lung tissues from collagen and epinephrine-injected mice. Treatments include control (WT:WT) (A), VF:WT (B), VF:WT + NAC (C), and VF:WT + aspirin (D). Arrows represent thrombi. P = .01, log-rank test. n = 3-4 mice per group, original magnification ×20. Shown are representative data from 2 biological replicates.