NAC reduces pulmonary thrombus formation in vivo. (A) Platelet counts in adult mice 1 hour before and 5 minutes following IV injection with a mixture of collagen (0.8 mg/kg) + epinephrine (6 mg/kg). (B) Kaplan-Meyer curve representing survival of mice injected with collagen + epinephrine mixture to induce a thromboembolism. Survival was recorded as 30 minutes after injection. (C) Quantification of pulmonary thrombi per image field. (D-G) Representative histology images of lungs from untreated control (D), collagen/epinephrine-treated (E), NAC + collagen/epinephrine (F), or aspirin + collagen/epinephrine (G). Pulmonary thrombi are indicated by black arrowheads; n = 6 mice per group, original magnification ×20.  *P < .05, **P < .01, ***P < .001, and ****P < .0001, 2-way ANOVA. Error bars represent SEM. Shown are representative data from 3 biological replicates.