CH in individuals. (A) Red bars represent individuals in MZ twin pairs; blue bars represent those in DZ pairs. Dark red/blue (lower stacks) depict the proportions with CH; pale red/blue (upper stacks) depict those without CH. Absolute numbers of individuals in each proportion are shown within each bar. There were no DZ individuals above the age of 80 years. (B) Distribution of the maximum VAF per individual among those with CH. VAFs are divided into 0.5% bins. (C) In the main grid, each column represents 1 individual, and each row 1 gene. If a grid square is colored, a mutation was detected, and the specific color indicates the mutation type (see key). The plot on the right shows the proportion of the cohort harboring a mutation in each gene. (D-E) Somatic variants identified in DNMT3A (D) and TET2 (E). Conserved/functional protein domains are colored red, and intervening domains gray. Each circle connected to the protein cartoon represents a mutation. Missense mutations are represented above and truncating mutations below the protein, with the color of the circle indicating specific mutation type (as per the key in panel C). (F) Distribution of the total number of mutations per individual. fs, frameshift.