FBXW7 mutations result in increased NOTCH1 target gene expression.PLAC8, NT5E, and CD27 expression was analyzed in (A) a cohort of NOTCH1 mutated (n = 28), FBXW7 mutated (n = 5), and non-NOTCH1/non-FBXW7 mutated (n = 174) CLL patients via gene expression analysis and (B) validated in another cohort of NOTCH1 mutated (n = 12), FBXW7 mutated (n = 12), NOTCH1 and FBXW7 mutated (n = 3), or nonmutated (n = 14) CLL cases via RT-qPCR. Details about NOTCH1 and FBXW7 mutations are listed in supplemental Table 5. Statistical significance was assessed via the Kruskal-Wallis test followed by the Dunn’s multiple comparison test (**P < .01; *P < .05). GEP, gene expression profiling.