Plasminogen promotes macrophage and dendritic cell accumulation at sites of inflammation. (A) FSC vs SSC plots for all genotypes represent all cell populations. Flow cytometry analysis of peritoneal lavage fluid at 72 hours after intraperitoneal thioglycollate injection shows decreased accumulation of total hematopoietic cells (B) and monocytes/macrophages (C) in the peritoneal cavity of plasminogen-deficient mice. The left panels in B and C are examples of flow cytometry of lavage fluid from individual mice. (D) Macrophages displayed differential expression levels of CD11b, Ly6C, CD86, and F4/80 markers. Thioglycollate stimulation significantly affected the large peritoneal macrophage (LPM) population (E), whereas the SPM population was mildly affected (F). Data are shown as individual values, with means and standard errors indicated.