ARIEL affects the TAL1-induced regulatory program through the regulation of ARID5B expression. (A) The high-confidence ARID5B target genes that were bound by ARID5B protein and downregulated by ARID5B knockdown in Jurkat cells were previously determined13 and used as a gene set. GSEA was performed to analyze the overall correlation of the gene expression changes following the ARIEL knockdown in the same cells. Normalized enrichment score (NES) and P values are shown. (B) The RNA expression of ARID5B and each member of the TAL1 complex was measured by qRT-PCR after the shRNA knockdown of ARIEL in Jurkat cells. See Figure 2C legend for details. (C) Western blot analysis showing the protein expression of each member of the TAL1 complex in the Jurkat cells after the ARIEL knockdown. (D) The high-confidence TAL1 target genes bound by TAL1 and downregulated after the TAL1 knockdown in the Jurkat cells were used as a gene set. See panel A legend for details. (E) ChIP analysis was performed using a specific antibody against TAL1, GATA3, RUNX1, or control IgG in Jurkat cells after the knockdown of ARIEL using shRNAs (sh3 and sh5). The amount of genomic DNA in the ChIP and input samples was measured by qRT-PCR using specific primers targeting the ARID5B −135-kb enhancer locus. The percentage of the input is shown as the mean plus or minus SD of duplicate experiments.