ARIEL positively regulates ARID5B expression in T-ALL cells. (A-B) The number of copies of ARIEL transcripts in each subcellular fraction was measured by performing digital droplet qPCR in Jurkat (A) and MOLT-4 (B) cells and shown as arbitrary unit (A.U.). (C-D) Jurkat (C) and MOLT-4 (D) cells were transduced by lentivirus infection with either a control shRNA (shGFP) or an shRNA targeting ARIEL (sh3 and sh5). RNA expression of ARIEL, ARID5B, and neighboring genes (CDK1 and JMJD1C) was measured by qRT-PCR 3 days after the transduction and normalized to GAPDH expression. Expression values relative to the control shGFP sample are shown as the mean plus or minus SD of triplicate experiments. (E) The protein expression of ARID5B in the Jurkat and MOLT-4 cells was analyzed by western blot analysis after the shRNA transduction. α-tubulin was used as an internal control. (F) Jurkat cells were transduced with either a control shRNA (shGFP) or an shRNA targeting ARID5B (sh3 and sh7). See panel C legend for details.