Reduced level of HAP1 in ALL patient leukemic cells correlates with increased resistance to l-asparaginase. (A) The panel of primary leukemia cell samples (n = 6) isolated from patient’s peripheral blood samples were treated with different concentrations of l-asparaginase, and IC₅₀ values were calculated after plotting l-asparaginase dose-dependent survival of leukemic cells measured by Alamar blue assay. Ratios of the HAP1 vs actin levels in patient samples (n = 6) were determined following densitometry analysis of immunoblots using the National Institutes of Health Image J 1.61 software. Numbers by the symbols correspond to patient number in panel B, which shows patient information.