IRF8⁺ LMPP signature genes are barely expressed in DC lineage cells. (A) Single-cell RT-qPCR analysis of Irf8 and Gapdh in WT and Irf8^−/− LMPPs. Expression levels of Irf8 and Gapdh are expressed in −1 × Ct units. (B) A heat map showing z scores for the expression of LMPP subpopulation-specific genes. A total of 151 genes, significantly upregulated or downregulated in WT Irf8-transcript⁺ LMPPs compared with WT Irf8-transcript⁻ LMPPs, were selected by using the scRNA-seq data (1-way analysis of variance, P < .05). Irf8-transcript⁺ cells were defined as cells with Irf8 fragments per kilobase million >10. (C) Representative LMPP subpopulation-specific genes. IRF8-dependent or IRF8-independent genes are shown as violin plots. *P < .05 (1-way analysis of variance). (D) GSEA comparing hematopoietic populations for 110 Irf8-transcript⁺ LMPP signature genes.