![Reconstitution of hematopoietic stem and progenitor cells >16 weeks in Mpl−/−mice transplanted with Mpl−/−BM transduced to express 1 of the selected genes. (A) Contribution of donor cells (CD45.2+) on LSK cells (mean plus or minus SD; 2-tailed, unpaired Student t test with Welch correction [*P = .024]; compared with the negative control). (B) Contribution of LSK cells in the nucleated BM cells (mean plus or minus SD; 2-tailed, unpaired Student t test with Welch correction [**P = .001]; compared with the negative control). (C) Representative flow cytometric analysis of LSK cells on the expression of CD34 and CD135 to discriminate between LT-HSCs, ST-HSCs, and MPP, as indicated, from mice of the gene groups Epcr and Hoxa10 compared with the negative and positive controls. (D) Contribution of MPP, ST-HSCs, and LT-HSCs on the LSK cells of the different gene groups (positive control, n = 10; negative control, n = 9; Pbx1, n = 7; Epcr, n = 6; Hoxa10 and Mycn, n = 3; mean plus or minus SD; Student t test with Welch correction [*P ≤ .05; **P ≤ .01]) (2-way ANOVA [P = .07]; Epcr vs negative control). (E) Number of total MPP, (F) ST-HSCs, and (G) LT-HSCs per mouse in the different gene groups. (E-G) Positive control, n = 10; negative control, n = 9; Pbx1, n = 7; Epcr, n = 6; Hoxa10 and Mycn, n = 3; mean plus or minus SD; 1-way ANOVA with Dunnett multiple comparisons test; Epcr vs negative control LT-HSCs (##P = .0037 [Student t test; P = .08]).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/133/13/10.1182_blood-2018-03-837344/3/blood837344f3.png?Expires=1720442595&Signature=FZC2hJ7cFPb~KNA~rq74tlh3ZCrUuK27KfHkzSRwqJzPoUpzOe3GGDwTbEmceP61ccWAUjS-ivPny8GVl8LqS5M65pwb3fVVjuTYegwhSAtv4z2qwvhIY8msyz6Lcqpi8TLMG46OatLtSmDOOBgItZO~kAt3NMdGs4V4l7yYc3GDlr3kPkGho-5NIl44mS-t7Wu0m~oVHGvNMdK3-Q2a~m6op1WUNM6oJQOkwTOm5p1JJGnbeQMD1wib51kIVmfa0e-eAQ1XCTyGmlx8Yx~7hBWKvjoUo244O~NgIZphQDPLvf6KZjnzWWcSO4UoDodwtVSHZ2SWnLzJKY5wKRreeA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Reconstitution of hematopoietic stem and progenitor cells >16 weeks in Mpl−/−mice transplanted with Mpl−/−BM transduced to express 1 of the selected genes. (A) Contribution of donor cells (CD45.2+) on LSK cells (mean plus or minus SD; 2-tailed, unpaired Student t test with Welch correction [*P = .024]; compared with the negative control). (B) Contribution of LSK cells in the nucleated BM cells (mean plus or minus SD; 2-tailed, unpaired Student t test with Welch correction [**P = .001]; compared with the negative control). (C) Representative flow cytometric analysis of LSK cells on the expression of CD34 and CD135 to discriminate between LT-HSCs, ST-HSCs, and MPP, as indicated, from mice of the gene groups Epcr and Hoxa10 compared with the negative and positive controls. (D) Contribution of MPP, ST-HSCs, and LT-HSCs on the LSK cells of the different gene groups (positive control, n = 10; negative control, n = 9; Pbx1, n = 7; Epcr, n = 6; Hoxa10 and Mycn, n = 3; mean plus or minus SD; Student t test with Welch correction [*P ≤ .05; **P ≤ .01]) (2-way ANOVA [P = .07]; Epcr vs negative control). (E) Number of total MPP, (F) ST-HSCs, and (G) LT-HSCs per mouse in the different gene groups. (E-G) Positive control, n = 10; negative control, n = 9; Pbx1, n = 7; Epcr, n = 6; Hoxa10 and Mycn, n = 3; mean plus or minus SD; 1-way ANOVA with Dunnett multiple comparisons test; Epcr vs negative control LT-HSCs (##P = .0037 [Student t test; P = .08]).