Transcriptional analysis and PML NB reformation on ATRA treatment. (A) GSEA analysis for 50 hallmark gene sets in P/R and P/R-FLT3ki mice treated or not during 9 or 24 hours with ATRA. Only significant pathways were represented (with FDR.q.val <0.05) according to NES value. (B) PML NB reformation assessed by immunofluorescence analysis of mPML (green) and human PML/RARA (red) with DAPI (blue) in bone marrow cells of P/R and P/R-FLT3ki APL mice treated with ATRA after 2 days (scale bar, 10 µm). (C) GSEA analysis of the p53 pathway in P/R and P/R-FLT3ki mice treated during 9 or 24 hours with ATRA. NES and FDR are noted. FDR.q.val <0.05 are in bold.