Overview of mutations identified by targeted sequencing. (A) Bar graph showing the frequency of mutated patients per gene for all genes with detected variants. Bars are colored according to the functional category of the gene (supplemental Table 16). Mutation frequencies are shown above the bars (%). FLT3 variants include FLT3-ITD and FLT3-TKD. (B) Box plots showing the median, 25% quantile, and 75% quantile of the VAF for all genes mutated in >4 patients. The dashed line indicates 50% VAF that was expected for heterozygous mutations. VAFs were corrected for patient-specific chromosomal aberrations and sex bias. Genes were color coded according to their assigned category. FLT3-ITDs were not included in this analysis. (C) Pie chart showing the distribution of 113 KIT mutations in 89 patients for the most frequent codons and exons, with mutation frequencies shown as percentage.