CMML MSCs EVs have an antifibrinolytic impact. For each experiment, PBS was used as a negative control. CMML EVs are represented in red, HD EVs in green, and PBS in blue. Continuous curves represented mEVs, and dotted curves represented sEVs. *P ≤ .05, **P ≤ .005. (A) Fibrinolysis parameters: clot lysis time (minutes, time between 50% of the peak during the coagulation phase [T_Np50Up] and 50% of the peak during the lysis phase [T_Np50Down]), Np_max (the maximum number of protofibril per fibrin fiber), Np₉₇ (97% of the maximum number of protofibril per fibrin fiber and corresponding to 97% of clot formation), Np₅₀ (50% of the maximum number of protofibril per fibrin fiber and corresponding to 50% of clot formation), coagulation time (time at which the clot formation begins), and T_Np97. (B) Fibrinography curves of our fibrinolysis assay on CMML EVs and HD MSC EVs. In each condition, 0.5 pM TF and 80 ng/mL t-PA were added. (C) Comparison of clot lysis times between CMML and HD MSC EVs. A longer clot lysis time corresponds to a higher clot resistance to fibrinolysis. (D) Comparison of coagulation times between CMML and HD MSC EVs. (E) Comparison of T_Np97 between CMML and HD MSC EVs.