Multicolor flow cytometry for the identification and expression of CD47 on plasma cells pretreatment and posttreatment with CyBorD DARA. (A) Representative dot plots are shown to illustrate the gating strategy for the identification of CD138 plasma cells. Plasma cells were identified by surface expression of CD138. (B) Dot plots indicate the mean fluorescence intensity (MFI) of CD47 expression pretreatment and posttreatment on CD138 MM cells from the bone marrow (n = 5) (left) and PB (n = 5) (right). Lines between dots indicate paired samples. Wilcoxon matched pairs signed rank test and paired Student t tests were used to detect statistically significant differences between pretreatment and posttreatment samples; *P < .05.