CD37CAR T cells exhibit enhanced effector functions, including specific killing of CD19⁻U2932 cells. (A) A representative analysis of CD19 and CD37 expression in Jurkat, BL-41, and U2932 cells by flow cytometry. (B) Specific killing mediated by CD19CAR or CD37CAR T cells against BL-41 and U2932 cells after 7 hours of coculture at the indicated E:T ratios. Data represent mean ± standard deviation of triplicates. Data from 1 of 2 experiments are shown. (C) Detection of intracellular cytokine production in CD19CAR or CD37CAR T cells after coincubation with target cells BL-41 or U2932 for 24 hours at an E:T ratio of 1:2. Data from 1 of 2 experiments are shown. Data represent mean ± standard deviation of quadruplicates. (D) Detection of degranulation in CD19CAR or CD37CAR T cells after coincubation with target cells BL-41 or U2932 for 6 hours at an E:T ratio of 1:2. Data from 1 of 2 experiments are shown. Data represent mean ± standard deviation of quadruplicates. (E) Flow cytometric analysis of CD19 and CD37 on the surface of GFP⁺ U2932 cells 5 hours after coculture with mock T cells, CD19CAR T cells, or CD37CAR T cells at an E:T ratio of 10:1. *P < .05, **P < .01, ***P < .001, ****P < .0001, Student t test with Bonferroni correction.