Par-4 overexpression in TCL1 mice hinders expansion of malignant clones. Expansion of malignant clones was quantified in vivo by EdU incorporation. (A) Representative flow plots and histograms showing EdU incorporation by single, live, CD45⁺CD19⁺CD5⁺ splenocytes from TCL1 and Par-4 × TCL1 mice. (B) Quantification of EdU-positive leukemic cells in TCL1 (n = 8) and Par-4 × TCL1 (n = 5) mice. Results were analyzed using a 2-sample Student t test for significance with α set to 0.05 (P = .0100). FSC-A, forward scatter area; FSC-H, forward scatter height.