Generation of transgenic mice expressing human Par-4 (PAWR). (A) Composition of the linearized DNA construct used for pronuclear injection. The human Par-4 gene (PAWR) is placed upstream of the immunoglobulin heavy-chain promoter and Eµ enhancer. (B) PCR verification of the human Par-4 transgene in the genome of the founder line using primers specific for the human homolog. (C) Whole-cell lysates were made from the indicated tissues harvested from Par-4 transgenic and WT littermates and analyzed by immunoblot for Par-4 expression. Lysate from human CLL cells was included as a positive control. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.