Figure 3.
Figure 3. Mitochondrial ROS generation is not altered in Nox2-deficient mice. Washed platelets from male and female mice deficient in Nox2 (−/y or −/−) or WT littermates (+/y or +/+) were incubated with 10 µM of DHR1,2,3 (A-D) or MitoSox (E-H) for 25 minutes at 37°C, in the presence or absence of stimulation with convulxin or thrombin. Samples were analyzed by flow cytometry. Data are presented as mean ± standard error (n = 4 or 5 mice in each group). *P < .05 vs. WT (+/y or +/+) without convulxin/thrombin, $P < .05 vs. Nox2-deficient (−/y or −/−) without convulxin/thrombin, @P < .05 vs. WT (+/y or +/+) with convulxin (25 ng/mL)/thrombin (0.02 U/mL), #P < .05 vs. Nox2-deficient (−/y or −/−) with convulxin (25 ng/mL)/thrombin (0.02 U/mL), 2-way ANOVA with the Tukey test for multiple comparisons.

Mitochondrial ROS generation is not altered in Nox2-deficient mice. Washed platelets from male and female mice deficient in Nox2 (−/y or −/−) or WT littermates (+/y or +/+) were incubated with 10 µM of DHR1,2,3 (A-D) or MitoSox (E-H) for 25 minutes at 37°C, in the presence or absence of stimulation with convulxin or thrombin. Samples were analyzed by flow cytometry. Data are presented as mean ± standard error (n = 4 or 5 mice in each group). *P < .05 vs. WT (+/y or +/+) without convulxin/thrombin, $P < .05 vs. Nox2-deficient (−/y or −/−) without convulxin/thrombin, @P < .05 vs. WT (+/y or +/+) with convulxin (25 ng/mL)/thrombin (0.02 U/mL), #P < .05 vs. Nox2-deficient (−/y or −/−) with convulxin (25 ng/mL)/thrombin (0.02 U/mL), 2-way ANOVA with the Tukey test for multiple comparisons.

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