Nox2 deficiency does not alter platelet ROS production in male or female mice. Levels of intracellular ROS, detected by oxidation of CM-H₂DCF, were measured in the presence or absence of stimulation with convulxin or thrombin in washed platelets from male (A-B) and female (C-D) WT (+/y or +/+) or Nox2-deficient (−/y or −/−) mice. The fluorescent signal generated as the result of oxidation of CM-H₂DCF to CM-DCF is presented as fold change over the signal observed with platelets from sex-matched WT mice without thrombin or convulxin. Data are presented as mean ± standard error (n = 6 or 7 mice in each group). *P < .05 vs. WT (+/y or +/+) without convulxin/thrombin, ^$P < .05 vs. Nox2-deficient (−/y or −/−) without convulxin/thrombin, ^@P < .05 vs. WT (+/y or +/+) with thrombin (0.02 U/mL), ^#P < .05 vs. Nox2-deficient (−/y or −/−) with thrombin (0.02 U/mL), 2-way ANOVA with the Tukey test for multiple comparisons.