Figure 2.
![Figure 2. hbbe1.1 and alas2 are expressed in hmgcs1 mutant larvae. Whole mount ISH was performed to detect the expression of hbbe1.1 (n = 41 hmgcs1+/+ [sibling], n = 34 hmgcs1−/− [Vu57]), (A-B) or alas2 (n = 16 hmgcs1+/+ [sibling], n = 15 hmgcs1−/− [Vu57]), (C-D) at 26 hpf. Purple, expression of each gene in the ICM; arrowhead, areas of increased or abnormal expression. Total number of animals was achieved with a minimum of 2 biological replicates. Images were taken with a 10× optical lens at 6.3× objective zoom.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/3/8/10.1182_bloodadvances.2018024539/2/advances024539f2.png?Expires=1724941656&Signature=XSr2UAtXRo3X72CZelWYSXnwVU3QIsJMfvkHJ~4vjKhIsIE1w-kNrx6BU4Ctr7UOJCzordHuLjbYVxgYuWeBSvYVvHhOfP1zPW~qTKm8gbiLH--NRRcOlOu43mf~VZq3HIbsSmkRepia5skFlqiZ7HyK1pE8EBwVFpiP02SJdw31dJ0BywBAKy2R1YO8-B2DbEF9Vuh8xqz9ysxY4aPlu5NxQoScGXeUf~AIIiHibAiTOeyLe~azViJJOhUUQuJBY77z0-fq7rNdu5-towbn3uBBSeY9KNB03RIwj-JdI7UTQ6N9ZpALn9HSU57wx36fkAK2aPpXXH13eYFOxk8Evg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
hbbe1.1 and alas2 are expressed in hmgcs1 mutant larvae. Whole mount ISH was performed to detect the expression of hbbe1.1 (n = 41 hmgcs1+/+ [sibling], n = 34 hmgcs1−/− [Vu57]), (A-B) or alas2 (n = 16 hmgcs1+/+ [sibling], n = 15 hmgcs1−/− [Vu57]), (C-D) at 26 hpf. Purple, expression of each gene in the ICM; arrowhead, areas of increased or abnormal expression. Total number of animals was achieved with a minimum of 2 biological replicates. Images were taken with a 10× optical lens at 6.3× objective zoom.