TAP-dependent peptide compartmentalization wanes during moDC differentiation (A) TAP-dependent peptide compartmentalization of monocytes, imDCs, and mDCs. MFI values of the histograms are indicated (left). Compartmentalization was performed in the presence of adenosine triphosphate (ATP; red line) and adenosine diphosphate (ADP; blue filled) to control for unspecific accumulation. Percent transport of monocytes, imDCs, and mDCs normalized to MFI values of monocyte adenosine triphosphate samples (means ± 95% CI, n = 10). ***P ≤ .0001; Kruskal-Wallis test with Dunn’s correction for multiple comparisons. (B) TAP-dependent peptide compartmentalization during differentiation of monocytes to mDCs (1 of 4 similar experiments is shown [left]). Percent TAP-dependent peptide compartmentalization (means ± 95% CI, n = 8 [right]). ***P ≤ .0001; **P ≤ .001; Kruskal-Wallis test with Dunn’s correction for multiple comparison. (C) ICP47^AT565-mediated inhibition of TAP-dependent peptide compartmentalization (means ± 95% CI, n = 6) in mDC. For ICP47^AT565, a 50% inhibitory concentration value of 34 nM was calculated with a 95% CI from 24 to 46 nM. (D) Bright field images of monocytes, imDCs, and mDCs. Blue 4′,6-diamidino-2-phenylindole stain indicates the nucleus, whereas the white dashed line indicates the cell shape. Scale bars, 5 µm. (E) Intracellular TAP1 staining in monocytes, imDCs, and mDCs (means ± 95% CI, n = 5). (F) TAP1 immunoblotting of monocytes, imDCs, and mDCs. IT, isotype staining; ns, not significant.