Defective erythropoiesis in mice with pan-hematopoietic Zfp148 deletion. (A-C) Peripheral blood counts and indices from Zfp148^fl/fl and Vav1-Cre; Zfp148^fl/fl mice at 15 to 26 weeks of age. (D) Representative flow cytometric analysis profiles of bone marrow for erythroid progenitor maturation using anti-CD71 and anti-Ter119 antibodies in Zfp148^fl/fl and Vav1-Cre; Zfp148^fl/fl mice. (E) Dot plots depicting the percentage of live BM cells in non- and early erythroid cells (CD71⁻Ter119⁻), late proerythroblasts (CD71⁺Ter119⁻), early erythroid cells (CD71⁺Ter119⁺) and RBCs (CD71⁻Ter119⁺) from the flow analysis in panel D (n = 5). (F-G) Representative flow cytometric analysis profile for spleen as in panels D and E. (H-I) Recovery from phenylhydrazine (PHZ)-induced hemolytic anemia in male (n = 8) and female (n = 12) mice. Spun hematocrit levels from zfp148^fl/fl, Vav1-Cre, and Zfp148^fl/fl control littermates preceding and following a 2-day course of phenylhydrazine intraperitoneal injection. A baseline spun hematocrit was obtained prior to phenylhydrazine injection and then repeated on days 3, 4, 5, 6, and/or 7 following initial injection. Hgb, hemoglobin; MCH, mean cell hemoglobin; MCV, mean cell volume; WBC, white blood cell.