PGN-induced procoagulant responses are amplified by cytokine signaling. (A) Procoagulant signals from PGN-stimulated donor PBMCs (blue circles) can be transferred to PGN-naive recipient PBMCs (red squares) by soluble mediators through paracrine signaling. Individual (symbols) and median responses (columns) are illustrated, depicting frequency of procoagulant monocytes (TF⁺CD14⁺; left panel) or monocyte TF fluorescence intensity (right panel). (B) Procoagulant responses induced by PGN (10 µg/mL) are sensitive to neutralization of paracrine and autocrine cytokine signaling. Pairwise representation of cytokine neutralization effect on the frequency of procoagulant monocytes induced by PGN (left panel) and normalized changes in PGN-induced monocyte TF antigen expression (right panel) in the presence of neutralizing antibodies are shown.