Gene expression profiling of megakaryocytes from single-KO and DKO mice. (A) Venn diagrams showing the numbers of genes up- and downregulated more than twofold in Pf4Sclc-KO, Lyl1-KO, and DKO megakaryocytes relative to WT mice (false discovery rate [FDR] <0.01). (B) Gene set enrichment analysis (GSEA) for platelet activation and aggregation genes between DKO and single-KO megakaryocytes. (C) Heat map showing expression of structural genes known to cause inherited macrothrombocytopenia. The FDR is shown for comparison of DKO with Pf4Sclc-KO cells. (D) Heat map showing expression of transcription factors that regulate megakaryopoiesis. FDR is shown for comparison of DKO and Pf4Sclc-KO cells. NES, normalized enrichment score; NS, not significant.