Platelet function tests of single-KO and DKO mice. (A) Tail bleeding times of mice. Mean ± SEM shown for 5 mice per genotype. Analyzed using two-way ANOVA with Tukey’s correction. (B) Surface platelet expression of P-selectin after stimulation with varying concentrations of thrombin. (C) Surface platelet expression of P-selectin after stimulation with thrombin, calcium ionophore (A23187), and collagen-reactive peptide (CRP). (D) Surface expression of glycoprotein 1b-α (Gp1ba) and glycoprotein 2b (Gp2b) on resting platelets. Mean ± SEM for 3 mice per genotype. **P < .001 compared with WT; ***P < .0001 compared with WT mice; ###P < .0001 compared with Pf4Sclc-KO. MCF, mean cell fluorescence.