Analysis of SLAMF7^high CD16⁻ monocytes in the PB of HCs and MPN patients with MF. (A) To define SLAMF7^highCD16⁻ monocytes, we began by gating lymphocyte subsets in a forward scatter/side scatter dot plot. Subsequently, we gated CD14⁺CD66b⁻ monocytes and divided them into SLAMF7^highCD16⁻, SLAMF7^lowCD16⁻, and CD16⁺ subsets. (B) The representative flow cytometry dot plot shows that the percentage of SLAMF7^highCD16⁻ monocytes increased in MPN patients with MF who harbored JAK2V617F compared with HCs. The ratios of the SLAMF7^highCD16⁻ subset to CD14⁺CD66b⁻ monocytes are presented as percentages.